MFT3 mAb reacted with CD43 gene products.

293 cells were stably transfected with CD43 complementary DNA (cDNA), stained with MFT3 (A) or S7 (B) mAb followed by FITC-MAR18.5, and analyzed on a FACScan. The shaded histogram shows results from staining with the first mAb; and the open histogram, results from staining without the first mAb. CD43 transfectant 293 cell lines were pretreated with 1B11 (C) or S7 (D), washed, stained with biotinylated MFT3 mAb followed by FITC-streptavidin, and analyzed on a FACScan. The shaded histogram shows results after pretreatment with mAbs; and the open histogram, results with no treatment. CD43 transfectant 293 cell lines were treated with 100 mU/ml neuraminidase, washed, and stained with MFT3 (E) or 1B11 (F) followed by FITC-MAR18.5. The shaded histogram show results after neuraminidase treatment; and the open histogram, results without neuraminidase treatment.