Fig. 2.
Fig. 2. Biochemical characterization of MFT3 antigen as the 130-kd CD43 glycoform. / (A) Lysates of bone marrow cells from BALB/c mice were subjected to sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE; 6% polyacrylamide gels), and Western blotting was performed with MFT3 or S7 (anti-115-kd CD43 glycoform) mAb. (B) MFT3-positive T-cell clones (KGU139) were lysed and immunoprecipitated with MFT3 mAb, subjected to SDS-PAGE (6%), and transferred to a nitrocellulose membrane. The membrane was probed with MFT3, 1B11 (anti-130-kd CD43 glycoform), or S7 mAb.

Biochemical characterization of MFT3 antigen as the 130-kd CD43 glycoform.

(A) Lysates of bone marrow cells from BALB/c mice were subjected to sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE; 6% polyacrylamide gels), and Western blotting was performed with MFT3 or S7 (anti-115-kd CD43 glycoform) mAb. (B) MFT3-positive T-cell clones (KGU139) were lysed and immunoprecipitated with MFT3 mAb, subjected to SDS-PAGE (6%), and transferred to a nitrocellulose membrane. The membrane was probed with MFT3, 1B11 (anti-130-kd CD43 glycoform), or S7 mAb.

Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal