CLP36 colocalizes with α-actinin but not with vinculin in human endothelial cells.

Shown are double-immunofluorescence micrographs of resting confluent cells (Ai-ii) and migrating cells (Bi-Ci). Note the colocalization of CLP36 (Aii,Bi,Cii) and α-actinin (Ai,Bii) along stress fibers (Biii) and the absence of CLP36 from focal adhesions (arrow in Cii) stained with antivinculin antibody (Ci). Bars, 5 μm (Aii,Bii,Cii).