IL-13 and IL-13Rα1 expression in Hodgkin lymphoma.

In situ hybridization for IL-13 (A, B) and IL-13Rα1 (C, E, F); black-colored silver grains denote mRNA expression. HRS cells from a case of mixed cellularity HL (A) express IL-13, whereas an L&H cell (arrow) from a case of NLPHL (B) is negative for IL-13. HRS cells from 2 cases of nodular sclerosis HL (C, E) express IL-13Rα1, whereas HRS cells from a case of mixed cellularity HL (F) are negative for IL-13Rα1. Serial sections from one of the cases of nodular sclerosis (C, D) were hybridized with an IL-13Rα1 antisense probe (C) or stained for CD30 (D), illustrating IL-13Rα1 expression in CD30⁺ HRS cells. Note IL-13Rα1 expression in surrounding lymphocytes and histiocytes (C, F).