Oligoclonal disease in primary mice with p190-induced B-lymphoid leukemia, with clonal restriction in secondary recipients.

Genomic DNA from lymphoblasts from pleural effusion (E) or lymph node (L) from a primary diseased animal (1°) or secondary transplant recipients of lymph node-derived lymphoblasts (2°) were digested withBglII and hybridized with a radioactive probe from the neomycin resistance gene. Groups of primary and secondary recipients are indicated by brackets; in some cases, 2 different secondary recipients of the same primary leukemia are shown. Tumor cell DNA from secondary transplant recipients often contained only a subset of the proviral clones observed in the primary tumor, suggesting that secondary transplantation selects for growth of particular clones, perhaps reflecting continued evolution of p190-transformed lymphoid cells to a more aggressive state. Control DNA (C) from a cell line containing a single BCR/ABL provirus is on the left, as are molecular size markers in kilobases (kb).