Fig. 1.
![Fig. 1. SH2 mutation decreases Bcr/Abl tyrosine kinase activity in vitro. / Bcr/Abl proteins were expressed by transfection of 293T cells and labeled in vivo with 35[S]-l-methionine. Lysates were immunoprecipitated with anti-Abl antisera and immune complexes incubated with γ-32[P]-ATP and GST-Crk substrate, as described.5 (A) 35[S] label, indicating relative levels of expression of the different Abl proteins. (B) 32[P] label. The position of the GST-Crk substrate is indicated by the arrowhead. The kinase activity of the different Bcr/Abl proteins relative to c-Abl after correction for levels of expression is shown at the top. (C) Coomassie blue stain demonstrating equal amounts of the GST-Crk substrate in all reactions.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/97/1/10.1182_blood.v97.1.4/6/h80110554001.jpeg?Expires=1724183941&Signature=tN0UTLCZssoqkrgHtLxY2Q~6bJLF1cmHiCgXzARHqdwTIbZhsWbcKOoiaGezqC7s4vQ4bZUxeqTdDdnzMRFPZnrWwYJX0dqk4ExkMtQ2AKicvksp67AUWlzOjSnIbX3sVeO6I74cWhdJYUSPWYrw6-kmY7lEHMKPawHfIhGfNF1wMypw4MvWs0SdMFpJrnARF7OfB2eyALomllJx0IWrcZGgI5Gq6GzdnBO1MxVugYXmTMnOI2MAbGiF7RmCHS6IyF0hdbOYc~psWxsPcVCibo~kFOVzk9X--hQzSm~oQ4c09rjf6vM7qGEhi7~BP1aPhdd0zH4-WIUJq5ZoZV7bMQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
SH2 mutation decreases Bcr/Abl tyrosine kinase activity in vitro.
Bcr/Abl proteins were expressed by transfection of 293T cells and labeled in vivo with 35[S]-l-methionine. Lysates were immunoprecipitated with anti-Abl antisera and immune complexes incubated with γ-32[P]-ATP and GST-Crk substrate, as described.5 (A) 35[S] label, indicating relative levels of expression of the different Abl proteins. (B) 32[P] label. The position of the GST-Crk substrate is indicated by the arrowhead. The kinase activity of the different Bcr/Abl proteins relative to c-Abl after correction for levels of expression is shown at the top. (C) Coomassie blue stain demonstrating equal amounts of the GST-Crk substrate in all reactions.
