Fig. 1.
Induction of JAB/SOCS1/SSI-1 mRNA and protein expression in preactivated PBLs by IL-2.
(A) Cells were either left untreated or stimulated with IL-2 for 1, 4, or 24 hours. mRNA was extracted and analyzed by Northern blotting using32P-labeled JAB/SOCS1/SSI-1 cDNA as a probe. The blot was stripped and reprobed using pHe7 cDNA to control variations in loading. (B) Preactivated PBLs were either left untreated or stimulated with IL-2 for 1, 4, or 24 hours, and JAB/SOCS1/SSI-1 protein expression was analyzed by Western blotting of cell lysates.
