Fig. 2.
Competition binding of 125I-labeled I-309 to HUVECs.
(A) Cells were incubated for 2 hours at 4°C with 0.2 nmol/L125I-labeled I-309 in the presence of increasing amounts of unlabeled recombinant I-309 (2-200 nmol/L). Indicated are the numbers of total counts (cpm) versus increasing concentrations of I-309 (Log M). Shown is a representative experiment of 3 performed in triplicate. (B) Scatchard tranformation of the data shown in panel A.
