Southern blot analysis of TK PCR products of peripheral blood mononuclear cells from a representative patient of the clinical trial at time of GVHD (day 30 after allograft) during GCV treatment.

Lanes A-D: DNA samples extracted from peripheral blood mononuclear cells of the patient at time of GVHD (A), 2 days (B), 4 days (C), 11 days (D) after the beginning of the GCV treatment. Lane E: negative control (nontransduced primary T cells). Lane F: positive control (DNA from G1Tk1SvNa vector producer cells).