Fig. 5.
Fig. 5. PCR amplification of the truncated. / HSV-Tk gene from genomic DNA obtained from transduced and selected primary T lymphocytes. The PCRs were set up using primers (HTK8+ and HTK1−, 446 bp) that specifically amplified the spliced form of the HSV-Tk gene in the GCV-resistant CEM-Tk clones.

PCR amplification of the truncated

HSV-Tk gene from genomic DNA obtained from transduced and selected primary T lymphocytes. The PCRs were set up using primers (HTK8+ and HTK1, 446 bp) that specifically amplified the spliced form of the HSV-Tk gene in the GCV-resistant CEM-Tk clones.

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