CXCR4, VLA-4 and VLA-5 are expressed at similar levels by cells in G₀/G₁ and S/G₂/M.

Representative FACS histograms of intracellular CXCR4 (A), VLA-4 (B), and VLA-5 (C) staining in cultured CD34⁺G₀/G₁ (solid lines) and S/G₂/M (dotted lines) CB cells. G₀/G₁ and S/G₂/M cells were purified by Hst sorting after 5 days of culture (see legend to Figure 1) and were then stained with anti–CD34-FITC and anti–CXCR4-PE, or anti–CD34-PE and anti–VLA-4-FITC or anti–VLA-5-FITC. Solid and dotted histograms show the control data for the G₀/G₁ and S/G₂/M cells, respectively, stained with irrelevant isotype-matched control antibodies. The S/G₂/M cells showed a higher green autofluorescence than the G₀/G₁cells.