American Society of Hematology

Fig. 1.

$Fig. 1. Distribution of the total population of cultured CB cells in different phases of the cell cycle. / CD34+ CB cells were cultured for 5 days in serum-free medium containing TPO (1day) and FL + SF + IL-3 + IL-6 + G-CSF (4 days) and then stained with Hst and Py and analyzed by FACS using the gates shown. A representative profile is shown in panel A. Corresponding FACS profiles are also shown for the total cultured CB cells (B) and the isolated G0/G1 (C) and S/G2/M (D) fractions after restaining of the fixed cells with PI.$

Distribution of the total population of cultured CB cells in different phases of the cell cycle.

CD34⁺ CB cells were cultured for 5 days in serum-free medium containing TPO (1day) and FL + SF + IL-3 + IL-6 + G-CSF (4 days) and then stained with Hst and Py and analyzed by FACS using the gates shown. A representative profile is shown in panel A. Corresponding FACS profiles are also shown for the total cultured CB cells (B) and the isolated G₀/G₁ (C) and S/G₂/M (D) fractions after restaining of the fixed cells with PI.

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