CD30 activation does not induce NF-κB activation in ALCL cells.

Michel cells were transfected with a luciferase reporter containing NF-κB binding sites. Cells were incubated with the immobilized CD30 agonistic antibodies M67 or isotype control antibodies for 12 hours and assayed for luciferase activity. Transfected cells were also incubated with either PMA (20 ng/mL) or a media control under the same conditions and assayed for luciferase activity.