Release of sγc after activation of immune cells in vivo.
(A) Increase of sγc in sera of BALB/c mice after intraperitoneal injection of 10 μg staphylococcal enterotoxin B per mouse. Sera of 3 mice per time-point were analyzed for their sγc content by ELISA. Bars indicate the means of 3 mice. (B) Sγc concentrations in supernatants of lymph node cell cultures obtained from BALB/c mice during the early course of an infection with L major. Mice were infected with 2 × 106L majorpromastigotes subcutaneously into the right hind footpad. At the time-points indicated, the popliteal lymph nodes were obtained, and their cells (2 × 106 cells/mL) were cultured for 48 and 96 hours in the presence or absence of LmAg. Concentrations of sγc were determined by ELISA.
