Fig. 3.
Kinetics of release of the sγc into culture supernatants after stimulation of immune cells analyzed by ELISA.
(A) Th2 cells of the L major- specific clone L1/1 (4 × 106 cells/mL) were stimulated with PMA (500 ng/mL), conA (5 μg/mL), or soluble L major antigen (LmAg) (3 × 106/well). (B) B cells purified by MACS from spleens of BALB/c mice (1 × 106 cells/mL, 95%-97% purity) were stimulated with PMA (500 ng/mL) or LPS (10 μg/mL). (C) NK cells from C57BL/6 mice were purified using Dynabeads yielding purity greater than 90%. 2.5 × 105 cells/mL were stimulated with PMA or conA, as above. (D) Peritoneal exudate cells (1.5 × 106 cells/mL) of BALB/c mice were stimulated with PMA, conA, LPS as above, or B burgdorferi (10 spirochetes per macrophage).
