Visualization of natural and recombinant soluble forms of the membrane-bound γc by Western blot.

Lane 1: naturally occurring sγc concentrated and purified from serum of BALB/c mice by affinity chromatography using the 4G3 mAb. Lane 2: Rsγc (255N-Stop-sγc) expressed in 293/EBNA cells. Lane 3: Rsγc expressed in E coli. Molecular mass markers are indicated on the left.