Analysis of the expression of various integrins on freshly isolated c-Fms⁺RANK⁻ cells or cultured cells.

(A) Freshly isolated c-Fms⁺RANK⁻ cells or colony cells derived from c-Fms⁺RANK⁻ cells cultured with M-CSF and sRANKL for 6 days were stained with anti-α_vβ₃, anti-α₂, anti-β₁, and anti-α₅ integrin antibodies or anti-RANK antibody, and expressions of integrins were analyzed by FACS. Red lines show the expression of integrins or RANK, and black lines show the isotype-matched control. (B) Expressions of α_v, β₃, α₂, β₁, and α₅ integrins were examined by RT-PCR in cells cultured in liquid or methylcellulose for 6 days in the presence of M-CSF and sRANKL.