Western blot analysis of WASP in blood lymphocytes of patients no. 2 and 6 and normal donor (control).

Protein extracts (40 μg) from patients no. 2 and 6 or from a normal donor T lymphocytes were separated by SDS-PAGE followed by transfer to PVDF membranes. Actin and WASP were detected using a monoclonal anti-WASP antibody (a gift from D. Nelson) and a monoclonal anti-β actin antibody (Sigma), respectively, and immunoreactive bands were visualized using an ECL detection system.