Effect of AA, LPA, and lyso-PC on EC migration.

BAECs were placed on the upper compartment of a Boyden chamber. AA (10 μmol/L), LPA (0.5 μmol/L), and lyso-PC (1 μmol/L) were placed in the lower compartment. Migration was carried out for 1 hour at 37°C. The number of migrated cells was determined as described in “Material and methods.” Bars represent the mean ± SE of 3 independent experiments. Asterisk indicates statistical significance (P < .005 versus control).