Fig. 7.
Effect of wild-type or H48Q mutant pancreatic sPLA2 on EC migration.
BAECs were placed on the upper compartment of a Transwell chamber. Wild-type porcine pancreatic sPLA2 (pGIB sPLA2) or a mutated pGIB sPLA2 (H48Q) were placed in the lower compartment of the Transwell chamber at concentrations of 1 μmol/L. Migration assay was carried out for 2 hours at 37°C. Quantitation of the migrated cells was performed by counting nuclei from 4 random fields (× 40) for each filter. Bars represent the mean ± SE from triplicate determination of a representative experiment. Asterisk indicates statistical significance (P < .001 versus wild-type pGIB sPLA2-induced migration).
