Effect of human synovial fluid on EC migration.

BAECs were placed on the upper compartment of a Transwell chamber. Various amounts of human synovial fluid (1-15 μL) were placed in the lower compartment containing 0.3 mL of HBSS medium. Migration assay was performed for 2 hours at 37°C. Migrated cells were quantitated by counting nuclei from 4 random fields (× 10)/filter as described above. Bars represent the mean ± SE of 3 independent experiments. Asterisk indicates statistical significance (P < .001 versus control, with no added human synovial fluid); HSF, human synovial fluid.