Fig. 3.
Fig. 3. Effect of different sPLA2s on EC migration. / BAECs were seeded in the upper compartment of a Transwell chamber. Bee venom sPLA2, Naja naja sPLA2, porcine pancreatic sPLA2, and OS1 (0.5 μmol/L each) were placed in the lower compartment. Cells that migrated after 2 hours of incubation at 37°C and 5% CO2 were stained. Nuclei from 4 random fields (× 10)/filter were counted. Results are the mean ± SE of the number of migrated cells per field from 3 independent determinations. Asterisk indicates statistical significance (P < .001 versus basal migration).

Effect of different sPLA2s on EC migration.

BAECs were seeded in the upper compartment of a Transwell chamber. Bee venom sPLA2, Naja naja sPLA2, porcine pancreatic sPLA2, and OS1 (0.5 μmol/L each) were placed in the lower compartment. Cells that migrated after 2 hours of incubation at 37°C and 5% CO2 were stained. Nuclei from 4 random fields (× 10)/filter were counted. Results are the mean ± SE of the number of migrated cells per field from 3 independent determinations. Asterisk indicates statistical significance (P < .001 versus basal migration).

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