Stimulation of EC migration by sPLA₂: dose response

. (A) BAECs (2.5 × 10⁵) were placed in the upper compartment of a Transwell chamber. Varying concentrations of bvPLA₂ were placed in the lower compartment. Cells were allowed to migrate for 2 hours through 8-μm pores of polycarbonate filters coated with attachment factor and placed between the upper and the lower compartment. After migration, the upper part of the filter was washed to remove nonmigrated cells and the membrane was stained using Diff-Quick. Migrated cells were quantified by counting nuclei in 4 random fields (× 10)/filter. Results are the mean ± SE of 4 independent experiments. Asterisk indicates statistically significant (P < .004 versus basal migration). (B) Stimulation of EC migration by sPLA₂: time course. BAECs were placed in the upper compartment of the Transwell chamber, and bvPLA₂ (1 μmol/L) was placed in the lower compartment. Migration was evaluated at the indicated times. Quantification of migrated cells was performed as described above. Results are the mean ± SE of 3 independent determinations. Asterisk indicates statistical significance (P < .001 basal migration).