Expression of
cbfb in zebrafish embryos. (A) Northern blot hybridization with 32P-labeled cbfb cDNA. RNA quantities in each lane of the Northern blot are shown by ethidium bromide staining of the gel. (B-I) In situ hybridization with digoxigenin-labeled cbfb antisense RNA probe on embryos of 10 hpf (B), 10-somite/14 hpf (C,D for lateral and dorsal views), 18-somite/18 hpf (E), 21-somite/19.5 hpf (F), 24 hpf (G,H for lateral and dorsal views), and 2 days postfertilization (I). Expression ofcbfb in Rohon-Beard cells are labeled with black arrows, in lateral plate mesoderm and ICM with red arrowheads, in fin buds with black arrowheads, in trigeminal ganglion with blue arrowheads, and in the retina with blue arrows. Embryos are all oriented with anterior to the left and posterior to the right. For the lateral views, the dorsal side of the embryos is in the upper part of the picture and the ventral is lower. Embryos in the subsequent figures are all oriented the same way.
