Fig. 6.
Expression of CD83 in PMNLs activated with TNF-α and IFN-γ.
PMNLs were incubated with TNF-α (1 ng/mL) and IFN-γ (25 U/mL) for 18 hours. (A) Expression of CD83 on resting or activated PMNLs was analyzed by flow cytometry. White area represents PMNLs kept at 4°C for 18 hours; shaded area represents PMNLs activated with both TNF-α and IFN-γ for 18 hours. (B) Detection of CD83 mRNA by RT-PCR. Representative of 3 experiments with similar results.
