Binding of ¹²⁵I-labeled LARC to the cell surfaces of PMNLs.

(A) PMNLs were incubated with both TNF-α (1 ng/mL) and IFN-γ (25 U/mL) for 6 hours. Activated PMNLs were incubated with 0.05 nmol/L of¹²⁵I-labeled LARC in the presence of increasing amounts of unlabeled LARC (0.003-562.5 nmol/L), IL-8 (0.01-63 nmol/L), or MCP-1 (0.01-63 nmol/L). (B) Scattered plot analysis.K_d, equilibrium dissociation constant (nmol/L); R, LARC-binding sites per cell. Representative of 3 different experiments with almost identical results is shown.