Fig. 3.
Fig. 3. Detection of CCR6 mRNA in cytokine-activated PMNLs by in situ hybridization. / PMNLs were incubated with TNF-α (1 ng/mL) and IFN-γ (25 U/mL) for 6 hours. (A) Hybridized with FITC-labeled antisense cRNA probe. Cytoplasm of PMNLs was stained positive for CCR6 mRNA. (B) Hybridized with FITC-labeled sense probe used as negative control. Magnification, 400×.

Detection of CCR6 mRNA in cytokine-activated PMNLs by in situ hybridization.

PMNLs were incubated with TNF-α (1 ng/mL) and IFN-γ (25 U/mL) for 6 hours. (A) Hybridized with FITC-labeled antisense cRNA probe. Cytoplasm of PMNLs was stained positive for CCR6 mRNA. (B) Hybridized with FITC-labeled sense probe used as negative control. Magnification, 400×.

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