Variations in Apaf-1 and procaspases in various pretreatment bone marrow samples.
(A) Aliquots containing 5 × 105 marrow mononuclear cells (at least 80% blasts) from patients with newly diagnosed AML (lanes 5-14) were subjected to SDS-PAGE, followed by blotting with reagents that recognize the indicated polypeptide. To provide a standard curve, aliquots containing 5 × 105, 2.5 × 105, 1.25 × 105, and 0.5 × 105 untreated HL-60 cells (lanes 1-4, respectively) were loaded onto each blot. The response of each patient to the first cycle of induction therapy is denoted above each lane as follows: − indicates NR; + indicates CR; 0 indicates UE. (B) Aliquots containing 5 × 105 mononuclear cells (lanes 5 and 7) or granulocytes (lanes 6 and 8) from the same AML marrows were analyzed as in panel A. Differential counts in each sample are indicated above each lane.
