Induced expression of CD86 and ICAM-1 mRNA in a cell-type–specific and time-dependent manner.

(A) HL60 and U937 cells were incubated with SB for the indicated time, and transcripts of CD86 and ICAM-1 were quantitated by real-time PCR method. Each real-time PCR was performed in quadruplicate, and the average fold induction relative to time 0 was shown with SD. (B) De novo protein synthesis is not required for the SB-induced expression of CD86 and ICAM-1 mRNA. HL60 cells were incubated for 6 hours with 0.7 mmol/L SB in the presence or absence of 10 μg/mL CHX.