American Society of Hematology

Fig. 3.

$Fig. 3. Adherent cells in LTC from newborn femur (A-D) and features of hematon preparations from young adult mice (E-H). / Cultured marrow from newborn femur contains several adherent cell types but lacks hematopoietic foci containing CA. (A) Osteocalcine-positive osteoblasts surrounded by hematopoietic cells. (B) A tartrate-resistant acid phosphatase–positive giant, multinuclear osteoclast surrounded by macrophages. (C) Cell island containing MMP-9/gelatinase-B–positive cells. (D) Firmly adherent αSM-actin–positive stroma cells at day 14 in culture. In young adult marrow, hematons resemble a bunch of grapes around capillaries after removal of the bulk buffy coat suspension. (E) Stereomicroscope view. (F) Phase-contrast microscope view. (G) Purified fraction of the hematon core complex. (H) More than 90% of core complexes develop in native CA at days 2 to 7 in culture.$

Adherent cells in LTC from newborn femur (A-D) and features of hematon preparations from young adult mice (E-H).

Cultured marrow from newborn femur contains several adherent cell types but lacks hematopoietic foci containing CA. (A) Osteocalcine-positive osteoblasts surrounded by hematopoietic cells. (B) A tartrate-resistant acid phosphatase–positive giant, multinuclear osteoclast surrounded by macrophages. (C) Cell island containing MMP-9/gelatinase-B–positive cells. (D) Firmly adherent αSM-actin–positive stroma cells at day 14 in culture. In young adult marrow, hematons resemble a bunch of grapes around capillaries after removal of the bulk buffy coat suspension. (E) Stereomicroscope view. (F) Phase-contrast microscope view. (G) Purified fraction of the hematon core complex. (H) More than 90% of core complexes develop in native CA at days 2 to 7 in culture.

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