Fig. 8.
Effect of neomycin sulfate on stimulation by mSLF, or immobilized anti–c-Kit antibodies.
(A) 32D cells expressing KitWT were incubated on X9/D3 cells or (B) on immobilized ACK-2 anti-Kit antibodies as described in “Materials and methods” in the presence of varying concentrations of neomycin sulfate for 18 hours, followed by a 6 hours pulse with3H-thymidine. Cells were then harvested and incorporated counts were determined by scintillation counting. Percentage control refers to incorporated counts observed in the absence of neomycin sulfate. Error bars represent the standard error determined from triplicate measurements.
