Fig. 5.
Neomycin sulfate inhibits stimulation of KitYF719- but not KitWT- or KitYF728-expressing cells.
32D cells infected with KitWT (▪), KitYF719 (●), or KitYF728 (▴) were incubated with 150 ng/mL sSLF and varying concentrations of neomycin sulfate for 18 hours at 37°C, followed by a 6 hours3H-thymidine pulse. Cells were then harvested and incorporated counts were determined by scintillation counting. Percentage control refers to incorporated counts observed in the absence of neomycin sulfate. Error bars represent the standard error determined from triplicate measurements.
