Fig. 4.
Stimulation of 32D infectants with sSLF.
(A) 32D cells infected with KitWT (○), KitYF719 (●), KitYF728 (■), KitYF719/YF728 (▪), or uninfected (×) were incubated with various concentrations of sSLF for 18 hours at 37°C, followed by a 6 hours 3H-thymidine pulse. Cells were then harvested and incorporated counts were determined by scintillation counting. Error bars represent the standard error determined from triplicate measurements. (B) 32D infectants were incubated with sSLF overnight. Percentage (%) viability was determined by scoring ability to exclude trypan blue. Viability of all cells in the presence of WEHI cm was 93% to 97%.
