SLF-stimulated mobilization of Ca⁺⁺ in 32D infectants.

The 32D cells infected with KitWT (A), KitYF719 (B), or KitYF728 (C), or uninfected (D) were loaded with 10 μmol/L INDO-1 for 1 hour at 37°C, followed by analysis by FACStar plus for Ca⁺⁺mobilization. 200 ng/mL SLF was added after 30 seconds to INDO-1 loaded cells. Ca⁺⁺ mobilization was followed over time as a ratio of bound Ca⁺⁺ signal/unbound Ca⁺⁺signal.