Effect of human sCD40L on VEGF-expression in vivo.

Immunohistochemical staining of VEGF in vivo in representative human skin transplants on SCID mice following injection of Matrigel without (panels A and C) or with (panels B and D) human sCD40L. Representative areas of panels A and B (200 × magnification) are viewed at high-power magnification (800 ×) in panels C and D, respectively. Consistently, there was enhanced expression of VEGF in sCD40L-treated human skins in multiple cells. There was no staining of the specimens with medium alone or isotype control antibody. Panel E represents the negative control staining of the same sample as in Panel D (800 ×).