Fig. 2.
![Fig. 2. Northern blot analysis. / Ten micrograms of total RNA sample were electrophoresed on agarose formaldehyde gels and transferred onto nylon membranes. The blots were hybridized with [α-32P] dCTP-labeled cDNA probes at 65°C overnight using a standard method. Filters were rinsed to a final stringency of 0.25 × SSC and exposed to a Kodak X-Omat or a Biomax film at -70 °C with an intensifying screen. After sequential stripping of the probes by a standard method, the blot was rehybridized with a GAPDH probe as a control.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/96/12/10.1182_blood.v96.12.3953/5/h82200348002.jpeg?Expires=1722765229&Signature=hLWxUgmGZjKpXGZiw1REB04UqzGy1C5hrIb2ktG8pMwR3cjCB9H91uvKpOScfTDENm5r643dl0OcEH0o6HJ6q4cA5k6BLrY0hfjnfTS9b~po8NHI7B9Cah2bkYDjFdmK7Ctp5YGG0ERb0E8FO8sirkNG3eQWw8yf4dQff4YgamYSuLKpxdFMmPpy2YPaa7XpVCBdOpTQOgpJ-oX~QK5Kw5FgdNU5xP0ykoAyQhcw~rpnjeQTR8Ci1ofYWrm4tuEroM74A6UzObFbb9xHZn0K~aSQpKr-e0nSpbZB3~ySWUjto5XN4HeNBn7qYmkmaGEat9gIaE7vi7X2tlEkXYm9CA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Northern blot analysis.
Ten micrograms of total RNA sample were electrophoresed on agarose formaldehyde gels and transferred onto nylon membranes. The blots were hybridized with [α-32P] dCTP-labeled cDNA probes at 65°C overnight using a standard method. Filters were rinsed to a final stringency of 0.25 × SSC and exposed to a Kodak X-Omat or a Biomax film at -70 °C with an intensifying screen. After sequential stripping of the probes by a standard method, the blot was rehybridized with a GAPDH probe as a control.
