![Fig. 1. Virtual Northern blot analysis. / Total RNAs were extracted from cells in the peritoneal lavage fluid. cDNAs were synthesized and polyclonally amplified. Five hundred nanograms of PCR-amplified (nonsubtracted) cDNAs were electrophoresed on agarose gels (1.2%), and Southern blotted onto nylon membranes. These filters were hybridized at 65°C for 3 hours using Rapid-hyb buffer (Amersham Life Science) with [α-32P] dCTP-labeled cDNA probes. Filters were rinsed to a final stringency of 0.25 × SSC and exposed to a Kodak X-Omat or a Biomax film at −70°C with an intensifying screen. Probes used in this study were generated from the cDNA library made by RDA. GAPDH was used as a control of expression. Size markers on the left expressed in kilobase. GenBank accession nos. BE846999, BE8470001, and BE847000 for unknown #1, #2, and #3, respectively.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/96/12/10.1182_blood.v96.12.3953/5/h82200348001.jpeg?Expires=1724148414&Signature=kOEfuLg0Z94SQ~1A-9MmzUXvCqcXsJCP6baSEfHSrMPFntfzYVaue2-y5-XI6jBVnfybWqp21CucU9vSkEOfnKgoggA2stq33SnzYH~2bVJMurDgBUd~gAJZ9F-YbLbnVeoiYsODuz45OwXPzwyM3QfDTdZDT3P6ODnSY3~ViSWR2~m54EoQRL-A9J1f8EuSrlNEecjq5JaLrqh53l-nx26r9Y-5rxci3sBkdeNBaNnY-Yx9MRVDA6F8U9j9-JaUnWGwzDKMweOPBnSUFZfb~F3kcM0Uk3ygy2-4IluCH8M1ciCgeUHx9i7VfZQm3iwz5AfPg-D2qbPQHsuBjd8bgQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Virtual Northern blot analysis.
Total RNAs were extracted from cells in the peritoneal lavage fluid. cDNAs were synthesized and polyclonally amplified. Five hundred nanograms of PCR-amplified (nonsubtracted) cDNAs were electrophoresed on agarose gels (1.2%), and Southern blotted onto nylon membranes. These filters were hybridized at 65°C for 3 hours using Rapid-hyb buffer (Amersham Life Science) with [α-32P] dCTP-labeled cDNA probes. Filters were rinsed to a final stringency of 0.25 × SSC and exposed to a Kodak X-Omat or a Biomax film at −70°C with an intensifying screen. Probes used in this study were generated from the cDNA library made by RDA. GAPDH was used as a control of expression. Size markers on the left expressed in kilobase. GenBank accession nos. BE846999, BE8470001, and BE847000 for unknown #1, #2, and #3, respectively.
