Fig. 7.
Fig. 7. Double-labeling fluorescence microscopy of GPIIb/GPV and GPIIb/GPIbα expression on permeabilized cultured CD34+cells. / CD34+ cells were permeabilized on the day of collection (day 0) and double labeled with anti-GPIIb revealed by FITC-GAM and anti-GPV–Cy3 or anti-GPIbα–Cy3. Cells at day 7 were permeabilized and labeled with anti-GPIIb revealed by GAM-Cy3 and anti-GPIbα–Alexa 488 or anti-GPV revealed by an Alexa 488–conjugated secondary antibody. The arrows indicate cells that are CD41+ but GPIbα− or GPV−. Neg represents the fluorescence of cells incubated with FITC-IgG1, Cy3-IgG1, or Alexa 488–IgG1.

Double-labeling fluorescence microscopy of GPIIb/GPV and GPIIb/GPIbα expression on permeabilized cultured CD34+cells.

CD34+ cells were permeabilized on the day of collection (day 0) and double labeled with anti-GPIIb revealed by FITC-GAM and anti-GPV–Cy3 or anti-GPIbα–Cy3. Cells at day 7 were permeabilized and labeled with anti-GPIIb revealed by GAM-Cy3 and anti-GPIbα–Alexa 488 or anti-GPV revealed by an Alexa 488–conjugated secondary antibody. The arrows indicate cells that are CD41+ but GPIbα− or GPV−. Neg represents the fluorescence of cells incubated with FITC-IgG1, Cy3-IgG1, or Alexa 488–IgG1.

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