Cell surface protein and mRNA expression of GPV, GPIbα, and GPIIb during the first days of culture.

(A) Cell surface expression of GPV (▪), GPIbα (░), and GPIIb-IIIa (CD41, ■) was followed by flow cytometry (Figure 2) until day 4 of culture. Results are expressed as the percentage of positive cells and are the mean ± SEM of 3 separate experiments. (B) The presence of GAPDH (positive control), GPV, GPIbα, and GPIIb transcripts was assessed by RT-PCR analysis.