Survivin does not inhibit caspase-3 activity.

(A) SDS gel electrophoresis of purified mouse recombinant survivin. (B) Effect of murine survivin or human XIAP on caspase-3 activity by hydrolysis of the fluorogenic substrate, DEVD-AMC. (C) Western blotting of immunoaffinity-purified human survivin isolated from Jurkat T cells by chromatography on α-survivin-sepharose. (D) Effect of native human survivin on caspase-3 activity by hydrolysis of the fluorogenic substrate, DEVD-AMC. W, whole cell extracts.