PBMCs from all RhD-negative donors alloimmunized with RhD-positive RBCs proliferate in response to peptides from the sequence of the RhD protein.

PBMCs were obtained from representative alloimmune donors 2 (A), 8 (B), and 13 (C) and tested for their ability to proliferate against the panel of peptides spanning the RhD protein. In each case, all the peptides were tested on a single occasion, and the broken line indicates the level of proliferation assumed to represent a positive response (the higher of SI = 3 or cpm = 1000).