Fig. 4.
Expression of mitochondrial Raf-1 in BCR/ABL and p185ΔBCR/Bcl-2–coexpressing cells.
(A) Levels of HSP90 and subunit IV of COX were measured as control of loading and subcellular fractionation. (B) Phosphorylation of BAD in BCR/ABL (wild-type and mutant) and p185ΔBCR/Bcl-2–coexpressing cells. Cells were starved of both IL-3 and serum for 3 hours and then cultured in 32P-orthophosphate–containing medium for 3 hours. Cell lysates were immunoprecipitated with anti-BAD polyclonal antibody, and phosphorylation was assessed by autoradiography.
