Fig. 9.
NPM-ALK-Y156F-Y418F and NPM-ALK-Y664F retain the ability to activate PI 3-kinase.
Ba/F3 cells were stably transfected with WT-NPM-ALK, NPM-ALK tyrosine-to-phenylalanine mutants, as indicated, or pCDNA3 vector only. PI 3-kinase activity was measured by incubation of antiphosphotyrosine (4G10) immunoprecipitates with phosphatidylinositol and32P-γ-ATP. 32P-labeled–enzymatic products of PI 3-kinase were separated by TLC on a silica gel plate and visualized by autoradiography.
