NPM-ALK partially rescues Cos1 cells from Bad-induced apoptosis.

Cos1 cells were transfected with Xpress-tagged Bcl2 (xp-Bcl2) and Flag-tagged Bad (Flag-Bad), plus either empty vector or NPM-ALK. Three days after the transfection, both floating and attached cells were collected and living cells determined by trypan blue staining (A). Data represent values ± SD of at least 3 independent experiments. Expression of NPM-ALK (left panel), Bad (middle panel), and cotransfected Bcl2 (right panel) was demonstrated by immunoblotting (B).