Effect of Ly294002 on the proliferation of NPM-ALK–transformed cells.

Bcr-Abl–transformed Ba/F3 (A), NPM-ALK–transformed Ba/F3 (B), and parental Ba/F3 cells cultured in the presence of IL-3 (C) were incubated with LY294002 (●, 7 μmol/L; ▴, 10 μmol/L) or with DMSO as control (▪). The number of viable cells was determined by trypan blue staining at the days indicated. Results are representative of at least 3 independent experiments.