Immunoblots of lysates fromfyn^−/−,lyn^−/−, andfyn^−/−lyn^−/−platelets.

Murine whole-platelet lysates were immunoblotted with anti-Fyn, anti-Lyn, and anti-Btk antisera after 10% sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE). (A)Fyn^−/−platelets express no detectable Fyn but normal levels of Lyn. (B) Conversely, lyn^−/−platelets express normal levels of Fyn but not Lyn. (C)Fyn^−/−lyn^−/−express neither Fyn nor Lyn but normal amounts of Btk. The traces are representative of 5 to 20 experiments.