Effect of PP1 on CRP and thrombin-stimulated aggregation.

Platelets were incubated with 0.1% DMSO or PP1 for 5 minutes before the addition of the agonist. (A) Shape change and aggregation of wild-type murine platelets was measured in a Born aggregometer after stimulation with CRP (1.0 μg/mL) in the absence and presence of PP1 (1-10 μmol/L). (B) Aggregation of wild-type murine platelets to thrombin (0.1 U/mL) was measured in the absence or presence of PP1 (1-10 μmol/L). Arrowhead indicates the time of agonist addition. The experiment is representative of 3 experiments with similar results.