Transduction efficiency of CFU-GM colonies as detected by PCR analysis of individual colonies derived from cord blood CD34⁺ cells transduced with the PGK-GFP vector.

(A) PCR analysis of randomly picked individual CFU-GM colonies derived from CD34⁺ cells directly after overnight transduction. (B) PCR analysis of CFU-GM colonies from the bone marrow of primary NOD/SCID mice engrafted with PGK-GFP transduced CD34⁺cells. (C) PCR analysis of CFU-GM colonies derived from secondary transplant NOD/SCID mice transduced with the same vector. M indicates mock transduced; B, blank; G, human genomic DNA; P, positive control.