Multilineage reconstitution and GFP expression in human CD45⁺ cells from a representative NOD/SCID transplant recipient.

This mouse (5.3) received a cell dose of 400 000 lentivirally transduced CD34⁺ cells. Transduction was performed overnight in serum-free medium supplemented with MGDF, MOI = 100. Lymphoid and myeloid differentiation of human SRC in NOD/SCID mice is shown by CD19 and CD33/15 antibody staining, respectively (71% of human cells were CD19⁺ and 28% were CD15/33⁺). Both lymphoid and myeloid populations expressed GFP; 23% of the CD19⁺ cells expressed GFP and 41% of the CD15/33⁺ cells expressed GFP.