RT-PCR analysis of LMP2A transcripts.

RT-PCR analysis was done of LMP2A transcripts in RNA preparations from enriched CD19⁺ cells of healthy carriers H2 and H1 (lanes 1 and 2) and from PBMCs of patients P1 and P2, both of whom had active PTLD (lanes 3 and 4). In each case, 5 μg RNA was used as the template for amplification. Positive and negative controls were RNA preparations from B95.8 and CA46 cells, respectively.